Native human Prethrombin 2 protein (ab62535)
Key features and details
- Expression system: Native
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Native human Prethrombin 2 protein -
Biological activity
Activity was measured by clotting and/or chromogenic substrate assay, following conversion of prothrombin to thrombin. -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Preparation and Storage
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Alternative names
- Prothrombin
- Prothrombin precursor
- PT
- Serine protease
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Relevance
Prethrombin 2, the smallest single-chain immediate precursor of a-thrombin (corresponding to residues Thr272 to Glu579 in prothrombin), has one glycosylation site at Asn373 and four disulfide bonds, Cys293-Cys439, Cys348-Cys364, Cys493-Cys507, and Cys521-Cys551. alpha-Thrombin can be derived from prothrombin by two pathways, each of which generates different intermediate products. In one pathway, human prothrombin is converted relatively slowly to alpha-thrombin in the presence of factor Xa and Ca2+ ions by initial cleavage at residue (Arg) 271/(Thr) 272 with consequent production of fragment 1.2 and prethrombin 2. Prethrombin 2 is then cleaved at R 320 with consequent generation of alpha-thrombin. In the other pathway, initial cleavage at R 320 yields an intermediate product, meizothrombin. -
Cellular localization
Secreted, extra cellular space
Images
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SDS-PAGE - Native human Prethrombin 2 protein (ab62535)Novex 4-12% Bis-Tris
1 µg per lane
MOPS