Native Human IgG protein (ab98981)
Key features and details
- Expression system: Native
- Suitable for: RID, SDS-PAGE
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Product name
Native Human IgG protein -
Purity
96 % .
IgA and IgM levels are -
Expression system
Native -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Native -
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.
Constituent: 0.158% Ammonium bicarbonate
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ReconstitutionReconstitution in phosphate buffer, pH >7.0 containing 0.15M NaCl is recommended.
Images
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ELISA - Native Human IgG protein (ab98981)
The wells were coated with ab195576 at 0.5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 40 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. ab201484 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.