Native Human Collagen III protein (ab7535)
Key features and details
- Expression system: Native
- Suitable for: IP, ELISA
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Product name
Native Human Collagen III protein
See all Collagen III proteins and peptides -
Expression system
Native -
Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 3% Acetic acid
Images
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Western blot - Native Human Collagen III protein (ab7535) Image courtesy of Michaela Leyh by Abreview.All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : plus ab7535 reduced and denatured
Lane 2 : plus ab7535 native
Secondary
All lanes : Goat anti-mouse-HRP at 1/2000 dilution
The protein can be used reduced + denaturated (band size ~130 kDa) or native (two bands ~ 130kDa and ~300kDa) The native protein gives a brighter signal.PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.