Mouse TARC ELISA Kit (CCL17) (ab213474)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.5 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TARC ELISA Kit (CCL17)
See all TARC/CCL17 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 9.6% Inter-assay Sample n Mean SD CV% Overall 3 6.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.5 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 86 81% - 92% Cell culture media 98 93% - 101% Hep Plasma 81 76% - 84% EDTA Plasma 107 103% - 112% Cit plasma 101 95% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TARC ELISA Kit (CCL17) (ab213474) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TARC (CCL17) protein in cell culture supernatant, cit plasma, hep plasma, serum, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TARC (CCL17) with 2.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
TARC (also known as C-C motif chemokine 17) is a chemokine that functions through chemokine receptors CCR4 and CCR8. TARC is expressed in the thymus as well as by dendritic cells, keratinocytes and bronchial epithelial cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse TARC Capture Antibody 1 x 600µl 10X Mouse TARC Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse TARC Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent NBS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4. -
Tissue specificity
Expressed at high levels in thymus and at low levels in the lung, colon and small intestine. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A-152E5.3
- A152E53
- ABCD 2
see all -
Database links
- Entrez Gene: 20295 Mouse
- Unigene: 41988 Mouse
Images
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Other - Mouse TARC ELISA Kit (CCL17) (ab213474)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse TARC standard curve comparison data.Standard curve comparison between mouse TARC SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Example of mouse TARC standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse TARC standard curve in Sample Diluent 50BS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse TARC standard curve in Sample Diluent 5BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked TARC in mouse serum and plasma samples.The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native TARC in mouse serum and plasma samples.The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 91 pg/mL in serum, 25.4 pg/mL in plasma (citrate), 23.0 pg/mL in plasma (EDTA) and 25.4 pg/mL in plasma (heparin).
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Interpolated concentrations of native TARC in mouse splenocyte and lung supernatant samples.The concentrations of TARC were measured in duplicates, interpolated from the TARC standard curves and corrected for sample dilution. Undiluted samples are as follows: splenocyte supernatant 50%, and lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TARC concentration was determined to be 25 pg/mL in splenocyte supernatant and 756 pg/mL in lung supernatant.
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Sandwich ELISA - Mouse TARC ELISA Kit (CCL17) (ab213474)To learn more about the advantages of recombinant antibodies see here.
