Mouse SP-D ELISA Kit (ab240683)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.16 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse SP-D ELISA Kit
See all Surfactant protein D/SP-D kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 2.2% Inter-assay Sample n Mean SD CV% Serum 3 2.3% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.16 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 103% - 108% Serum 99 94% - 102% Tissue Extracts 86 83% - 88% Hep Plasma 105 99% - 115% EDTA Plasma 98 89% - 110% Cit plasma 100 89% - 102% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse SP-D ELISA Kit (ab240683) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SP-D protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse SP-D with 2.16 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse SP-D Capture Antibody 1 x 600µl 10X Mouse SP-D Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse SP-D Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. -
Sequence similarities
Belongs to the SFTPD family.
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsThe N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. -
Cellular localization
Secreted > extracellular space > extracellular matrix. Secreted > extracellular space > surface film. - Information by UniProt
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Alternative names
- COLEC 7
- COLEC7
- Collectin-7
see all -
Database links
- Entrez Gene: 20390 Mouse
- SwissProt: P50404 Mouse
- Unigene: 1321 Mouse
Images
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Other - Mouse SP-D ELISA Kit (ab240683)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse SP-D Standard Curve Comparison.Standard Curve comparison between mouse SP-D SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of mouse SP-D standard curve in Sample Diluent NS.The SP-D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse SP-D standard curve in 1X Cell Extraction Buffer PTR.The SP-D standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native SP-D in mouse serum and plasma samples.The concentrations of SP-D were measured in duplicates, interpolated from the SP-D standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 20%, plasma (citrate) 15%, plasma (EDTA) 30%, and plasma (heparin) 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 4172.67 pg/mL in serum, 5466.91 pg/mL in plasma (citrate), 3129.78 pg/mL in plasma (EDTA), and 2563.11 pg/mL in plasma (heparin). -
Interpolated concentrations of native SP-D in mouse cell culture supernatant samples.The concentrations of SP-D were measured in duplicates, interpolated from the SP-D standard curves and corrected for sample dilution. Undiluted samples are as follows: LPS treated lung supernatant 1%, and untreated lung supernatant 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 82.93 ng/mL in LPS treated lung supernatant and 26.10 ng/mL in untreated lung supernatant. -
Interpolated concentrations of native SP-D in mouse lung extract based on a 50 µg/mL extract load.The concentrations of SP-D were measured in duplicate and interpolated from the SP-D standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SP-D concentration was determined to be 929.77 pg/mL in lung extract. -
Sandwich ELISA - Mouse SP-D ELISA Kit (ab240683)To learn more about the advantages of recombinant antibodies see here.
