Mouse PDGFR beta ELISA Kit (ab253225)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 20.3 pg/ml
- Range: 109.4 pg/ml - 7000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PDGFR beta ELISA Kit
See all PDGFR beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.3% Inter-assay Sample n Mean SD CV% Serum 3 2.6% -
Sample type
Cell culture supernatant, Serum, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
20.3 pg/ml -
Range
109.4 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105 104% - 107% Serum 93 90% - 95% Cell culture media 104 103% - 106% EDTA Plasma 112 106% - 116% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse PDGFR beta ELISA Kit (ab253225) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGFR beta protein in cell culture media, cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PDGFR beta with 20.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
PDGFR beta (Platelet-derived growth factor receptor beta) is a tyrosine kinase receptor for members of the platelet derived growth factor family, homodimeric PDGFB and PDGFD, and heterodimeric PDGFA/B. Binding plays an essential role in embryonic development, blood vessel development, and cell proliferation, migration, survival, chemotaxis, and differentiation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse PDGFR beta Capture Antibody 1 x 600µl 10X Mouse PDGFR beta Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Mouse PDGFR beta Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2. -
Involvement in disease
Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Beta platelet derived growth factor receptor
- Beta-type platelet-derived growth factor receptor
- CD 140B
see all -
Database links
- Entrez Gene: 18596 Mouse
- SwissProt: P05622 Mouse
- Unigene: 4146 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse PDGFR beta standard curve in Sample Diluent NS.The PDGFR beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PDGFR beta in mouse serum, plasma (EDTA), and cell culture supernatant samples.The concentrations of PDGFR beta were measured in duplicates, interpolated from the PDGFR beta standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, NIH/3T3 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGFR beta concentration was determined to be 28.59 ng/mL in neat serum, 22.71 ng/mL in neat plasma (EDTA), and 0.578 ng/mL in neat NIH/3T3 cell culture supernatant.
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Sandwich ELISA - Mouse PDGFR beta ELISA Kit (ab253225)To learn more about the advantages of recombinant antibodies see here.
