Mouse PDGF BB ELISA Kit (ab224879)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.9 pg/ml
- Range: 9.375 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PDGF BB ELISA Kit
See all PDGF B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 4.2% Inter-assay Sample n Mean SD CV% Serum 5 5.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.9 pg/ml -
Range
9.375 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 111 104% - 115% Cell culture media 92 91% - 93% Hep Plasma 102 97% - 106% EDTA Plasma 101 94% - 111% Cit plasma 109 104% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse PDGF BB ELISA Kit (ab224879) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PDGF BB protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PDGF BB with 1.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
PDGF B (Platelet derived growth factor B) plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. As a homodimer it is a potent mitogen for cells of mesenchymal origin and is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGF-BB is essential for normal blood vessel development, for normal development of kidney glomeruli and in wound healing. Signaling is modulated by the formation of heterodimers with PDGF A chain to yield PDGF-AB.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml 10X Mouse PDGF BB Capture Antibody 1 x 600µl 10X Mouse PDGF BB Detector Antibody 1 x 600µl Mouse PDGF BB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Becaplermin
- c sis
- FLJ12858
see all -
Database links
- Entrez Gene: 18591 Mouse
- SwissProt: P31240 Mouse
- Unigene: 144089 Mouse
Images
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Other - Mouse PDGF BB ELISA Kit (ab224879)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse PDGF BB Competitor standard curve comparisonStandard curve comparison between Mouse PDGF BB SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse PDGF BB standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PDGF BB in mouse serum and plasma samples.The concentrations of PDGF BB were measured in duplicates, interpolated from the PDGF BB standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 4%, plasma (citrate) 25%, plasma (heparin) 25% and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF BB concentration was determined to be 9591 pg/mL in neat serum, 976.9 pg/mL in neat plasma (citrate), 555.5 pg/mL in neat plasma (heparin), and 1773 pg/mL in neat plasma (EDTA).
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Interpolated concentrations of native PDGF BB in mouse LPS stimulated J774A.1 cell culture supernatant (72 hours) samples.The concentrations of PDGF BB were measured in duplicates, interpolated from the PDGF BB standard curves and corrected for sample dilution. Undiluted samples are as follows: J774A.1 cell culture supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF BB concentration was determined to be 3882 pg/mL in neat J774A.1 cell culture supernatant.
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Other species reactivity was determined.Other species reactivity was determined by measuring 4% rat serum samples, interpolating the protein concentrations from the mouse standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in mouse serum assayed at the same dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGF BB concentration was determined to be 10,988 pg/mL in neat mouse serum and 1368.2 pg/mL in neat rat serum. Reactivity with rat serum was determined to be 12%.
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Sandwich ELISA - Mouse PDGF BB ELISA Kit (ab224879)To learn more about the advantages of recombinant antibodies see here.