Mouse PDGF AA ELISA Kit (ab277421)
Key features and details
- Sensitivity: 3.2 pg/ml
- Range: 3.28 pg/ml - 800 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Human
Overview
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Product name
Mouse PDGF AA ELISA Kit
See all PDGF AA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.2 pg/ml -
Range
3.28 pg/ml - 800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 141.1 137% - 145% Plasma 133.4 116% - 141% Cell culture media 135.2 123% - 146% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse, Human -
Product overview
Mouse PDGF AA ELISA Kit (ab277421) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse PDGF AA in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse PDGF AA coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse PDGF AA present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse PDGF AA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse PDGF AA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Mouse PDGF AA Antibody 2 vials Anti-Mouse PDGF AA coated Microplate 1 unit Mouse PDGF AA standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
The long form contains a basic insert which acts as a cell retention signal. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- PDGF A
- PDGF A chain
- PDGF subunit A
see all -
Database links
- Entrez Gene: 5154 Human
- Entrez Gene: 18590 Mouse
- Omim: 173430 Human
- SwissProt: P04085 Human
- SwissProt: P20033 Mouse
- Unigene: 535898 Human
- Unigene: 2675 Mouse
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.