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Mouse OX40 ELISA Kit (TNFRSF4) (ab253218)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 33 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse OX40 ELISA Kit (TNFRSF4)
    See all CD134 / OX40L receptor kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 8%

  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    33 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85 85% - 86%
    Cell Lysate 101 96% - 104%
    EDTA Plasma 98 95% - 100%
    Cit plasma 89 87% - 91%
    Tissue Lysate 101 98% - 104%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse OX40 ELISA Kit (TNFRSF4) (ab253218) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OX40 protein in cell culture supernatant, cell lysate, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse OX40 with 33 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    OX40, also known as TNFRSF4 and/or CD134, is a constitutively expressed transmembrane receptor found on T-cells. OX40 serves as a secondary checkpoint for immunological responses through the binding of TNF receptor associated factor (TRAF) proteins. OX40 and its predominant ligand, OX40L, regulate adaptive T cell immune responses. Clinically, OX40 is the target of several immunotherapy antibody treatments to promote antitumor immunity.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse OX40 ELISA Kit (ab253218)
    Other - Mouse OX40 ELISA Kit (ab253218)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of mouse OX40 standard curve in Sample Diluent NS.
    Example of mouse OX40 standard curve in Sample Diluent NS.

    The OX40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Example of mouse OX40 standard curve in 1X Cell Extraction Buffer PTR.
    Example of mouse OX40 standard curve in 1X Cell Extraction Buffer PTR.

    The OX40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native OX40 in mouse serum and plasma samples.
    Interpolated concentrations of native OX40 in mouse serum and plasma samples.

    The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (EDTA) 100%, and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 546.8 pg/mL in serum, 530.6 pg/mL in plasma (citrate), 522.3 pg/mL in plasma (EDTA), and 626.3 pg/mL in plasma (heparin).

  • Interpolated concentrations of native OX40 in mouse spleen supernatant sample treated with 2.5 µg/mL Concanavalin A for 72 hours.
    Interpolated concentrations of native OX40 in mouse spleen supernatant sample treated with 2.5 µg/mL Concanavalin A for 72 hours.

    The concentrations of OX40 were measured in duplicates, interpolated from the OX40 standard curves and corrected for sample dilution. Undiluted samples are as follows: spleen supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 564.3 pg/mL in spleen supernatant.

  • Interpolated concentrations of native OX40 in treated and untreated mouse splenocyte cell extract samples based on a 500 µg/mL extract load.
    Interpolated concentrations of native OX40 in treated and untreated mouse splenocyte cell extract samples based on a 500 µg/mL extract load.

    The concentrations of OX40 were measured in duplicate and interpolated from the OX40 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OX40 concentration was determined to be 2,184.1 pg /mL in treated splenocyte cell extract samples and 605 pg/mL in untreated splenocyte cell extract samples.

  • Sandwich ELISA - Mouse OX40 ELISA Kit (TNFRSF4) (ab253218)
    Sandwich ELISA - Mouse OX40 ELISA Kit (TNFRSF4) (ab253218)
    To learn more about the advantages of recombinant antibodies see here.

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