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Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

Key features and details

  • Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
  • Conjugation: Alkaline Phosphatase
  • Host species: Mouse
  • Isotype: IgG2b
  • Suitable for: ELISA, WB

Overview

  • Product name

    Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
    See all IgG Light chain secondary antibodies

  • Host species

    Mouse

  • Target species

    Rabbit

  • Specificity

    ab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD).

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Immunogen

    Rabbit Light Chain

  • Conjugation

    Alkaline Phosphatase

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 8.00
    Preservative: 0.1% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    SB62a

  • Isotype

    IgG2b

  • Light chain type

    lambda

  • Research areas

Images

  • Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)
    Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

    Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains

    Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)

    Lane 1: 10 µg rabbit monoclonal was loaded
    Lane 2: 5 µg rabbit monoclonal was loaded
    Lane 3: 2 µg rabbit monoclonal was loaded

    Right blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000

    Lane 1: 1 µg rabbit monoclonal was loaded
    Lane 2: 0.5 µg rabbit monoclonal was loaded
    Lane 3: 0.25 µg rabbit monoclonal was loaded

  • Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)
    Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)
    All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
    Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
    Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

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