Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)
![Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)](https://www.abcam.com/ps/products/99/ab99696/Images/ab99696-113788-mouse-monoclonal-sb62a-anti-rabbit-igg-light-chain-alkaline-phosphatase-western-blot.jpg "Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)")
Key features and details
- Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Mouse
- Isotype: IgG2b
- Suitable for: ELISA, WB
Overview
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Product name
Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)
See all IgG Light chain secondary antibodies -
Host species
Mouse -
Target species
Rabbit -
Specificity
ab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD). -
Tested applications
Suitable for: ELISA, WBmore details -
Immunogen
Rabbit Light Chain
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Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SB62a -
Isotype
IgG2b -
Light chain type
lambda -
Research areas
Images
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Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)
Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains
Left blot: Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)
Lane 1: 10 µg rabbit monoclonal was loaded
Lane 2: 5 µg rabbit monoclonal was loaded
Lane 3: 2 µg rabbit monoclonal was loadedRight blot: WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000
Lane 1: 1 µg rabbit monoclonal was loaded
Lane 2: 0.5 µg rabbit monoclonal was loaded
Lane 3: 0.25 µg rabbit monoclonal was loaded -
![Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)](https://www.abcam.com/ps/products/99/ab99696/Images/ab99696-113787-mouse-monoclonal-sb62a-anti-rabbit-igg-light-chain-alkaline-phosphatase-western-blot.jpg)
Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
