Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.87 pg/ml
- Range: 3.13 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MIP2 ELISA Kit (CXCL2)
See all CXCL2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% overall 8 1.64% Inter-assay Sample n Mean SD CV% overall 3 4.87% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.87 pg/ml -
Range
3.13 pg/ml - 200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108 103% - 113% Cell culture media 102 98% - 107% Hep Plasma 104 101% - 109% EDTA Plasma 115 113% - 116% Cit plasma 91 87% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse MIP2 ELISA Kit (CXCL2) (ab204517) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP2 (CXCL2) protein in cell culture supernatant, plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MIP2 (CXCL2) with 0.87 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse Macrophage Inflammatory Protein-2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 was originally identified as a heparin-binding protein, and has been shown to exhibit potent neutrophil chemotactic activity.
Mouse MIP2 c-DNA encodes a 100 amino acid residue precursor protein. The amino-terminal 27 amino acid residues are cleaved from this precursor to generate the mature mouse MIP2. Mouse MIP2 is 63% identical to Mouse KC (another mouse alpha chemokine), and mouse MIP2 is 60% identical to human GROβ and GROγ. Based on these protein sequence similarities, it is likely that mouse MIP2 and KC are homologs of the human GROα, β, and γ chemokines. However, since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse MIP2 and KC are functional homologs of human IL-8 in mice. A putative mouse homolog of the human IL-8 receptor beta (IL-8 Rβ) has also been cloned. This receptor shows 71% identity to human IL-8 Rβ and 68% identity to human IL-8 Rα. Both mouse MIP2 and KC bind mouse IL-8 Rβ with high affinity.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse MIP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Mouse MIP2 Capture Antibody (Lyophilized) 1 vial Mouse MIP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 2
- Chemokine (C X C motif) ligand 2
- Chemokine, CXC motif, ligand 2
see all -
Database links
- Entrez Gene: 20310 Mouse
- SwissProt: P10889 Mouse
- Unigene: 4979 Mouse
Images
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Other - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse MIP2 standard curve comparison data.Standard curve comparison between mouse MIP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit show comparable sensitivity.
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Linearity of dilution of MIP2 in serum and cell culture media.Recombinant mouse MIP2 was spiked into 100% serum and diluted in a 2-fold dilution series in Sample Diluent 50BP. Recombinant mouse MIP2 was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Linearity of dilution of spiked recombinant MIP2 in plasma.Recombinant mouse MIP2 was spiked into 100% citrate plasma and 50% EDTA plasma and diluted in a 2-fold dilution series in Sample Diluent 25BP. Recombinant mouse MIP2 was spiked into 100% heparin plasma and diluted in a 2-fold dilution series in Sample Diluent 50BP. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Mouse MIP2 expression is shown for serum and plasma samples.Native MIP2 was measured in duplicate in 100% serum and 100% heparin plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 50BP. Native MIP2 was measured in duplicate in 100% Citrate plasma and 50% EDTA plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 25BP. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Mouse MIP2 expression is shown for cultured media from two mouse cell lines.J774A.1 cells were cultured in HGDMEM with 10% fetal calf serum, and 100 µg/mL of Kanamycin. During the exponential growth phase, J774A.1 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. RAW 264.7 cells were cultured in HGDMEM with 10% fetal calf serum, 2 mM L-glutamine and 100 µg/mL Kanamycin. During the exponential growth phase, RAW264.7 cells were starved for 24 hours and treated in the presence and absence of 5 µg/mL of LPS (Batch #1) or 1% PHA (Batch #2).The concentrations of mouse MIP2 were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
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Example of the mouse MIP2 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - Mouse MIP2 ELISA Kit (CXCL2) (ab204517)To learn more about the advantages of recombinant antibodies see here.
