Mouse IL-20R2 ELISA Kit (ab277472)
Key features and details
- Sensitivity: 0.45 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-20R2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.45 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 78.01 69% - 92% Plasma 80.43 68% - 111% Cell culture media 109.3 80% - 138% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-20R2 ELISA Kit (ab277 in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse IL-20R2 in serum (mouse IL-20 R beta concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse IL-20R2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse IL-20R2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse IL-20R2 antibody is added. After washing away unbound biotinylated antibody, H472) is anRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse IL-20R2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Mouse IL-20 R beta Antibody 2 vials Anti-Mouse IL-20R2 coated Microplate 1 unit Mouse IL-20R2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
The IL20RA/IL20RB dimer is a receptor for IL19, IL20 and IL24. The IL22RA1/IL20RB dimer is a receptor for IL20 and IL24. -
Tissue specificity
Widely expressed with highest levels in skin and testis. Highly expressed in psoriatic skin. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- DIRS1
- Fibronectin type III domain containing 6
- FNDC6
see all -
Database links
- Entrez Gene: 213208 Mouse
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.