Mouse IL-15 ELISA Kit (ab275898)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 45.4 pg/ml
- Range: 131.3 pg/ml - 8400 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IL-15 ELISA Kit
See all IL-15 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5.1% Inter-assay Sample n Mean SD CV% Serum 3 5.53% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
45.4 pg/ml -
Range
131.3 pg/ml - 8400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82 80% - 84% Cell culture media 108 107% - 108% Hep Plasma 103 100% - 106% EDTA Plasma 83 79% - 86% Cit plasma 80 79% - 81% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-15 ELISA kit (ab275898) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse IL-15 protein in serum, plasma, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-15 with 45.4 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin (IL)-15 is a proinflammatory cytokine found expressed in a large number of cell types and tissues, and mainly secreted by mononuclear phagocytes. IL-15 acts as a pleiotropic cytokine that plays a vital role in the innate and adaptive immunity. Main function of IL-15 is to stimulate cell activation and proliferation of natural killer cells by signaling through IL-15 receptor and T-lymphocytes by interacting with IL-2 beta and gamma components.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IL-15 Capture Antibody 1 x 600µl 10X Mouse IL-15 Detector Antibody 1 x 600ml 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BR 1 x 6ml Mouse IL-15 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. -
Tissue specificity
Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. - Information by UniProt
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Alternative names
- IL 15
- IL-15
- IL15
see all -
Database links
- Entrez Gene: 16168 Mouse
- SwissProt: P48346 Mouse
- Unigene: 4392 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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. Example of mouse IL-15 standard curve in Sample Diluent NS + 1X Enhancer. -
Interpolated concentrations of spike IL-15 in mouse serum and plasma samples. -
Sandwich ELISA - Mouse IL-15 ELISA Kit (ab275898)To learn more about the advantages of recombinant antibodies see here.
