Mouse IL-10 Matched Antibody Pair Kit (ab214473)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 7.81 pg/ml - 500 pg/ml
Overview
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Product name
Mouse IL-10 Matched Antibody Pair Kit
See all IL-10 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.49 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IL-10 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IL-10.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse IL-10 Capture Antibody 2 x 50µg 1 x 50µg Mouse IL-10 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse IL-10 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
Database links
- Entrez Gene: 16153 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
Images
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Mouse IL-10 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse IL-10 Matched Antibody Pair Kit (ab214473)To learn more about the advantages of recombinant antibodies see here.
