Mouse IFNGR1 ELISA Kit (ab267580)
Key features and details
- Sensitivity: 0.16 ng/ml
- Range: 0.164 ng/ml - 40 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IFNGR1 ELISA Kit
See all IFNGR1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.16 ng/ml -
Range
0.164 ng/ml - 40 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108 102% - 115% Plasma 89 67% - 128% Cell culture media 87 79% - 93% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IFNGR1 ELISA Kit is designed for the quantitative determination of IFNGR1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse IFNGR1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IFNGR1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IFNGR1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFNGR1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit detects mouse IFN-gamma R1. No cross-reactivity with mouse IFNalpha/beta R1, mouse IFN-gamma R2, human IFN-gamma R1 is observed.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Mouse IFNGR1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse IFNGR1 Detection Antibody 2 vials Mouse IFNGR1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. -
Involvement in disease
Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated at Ser/Thr residues. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Antiviral Protein Type II
- Antiviral protein, type 2
- AVP type II
see all -
Database links
- Entrez Gene: 15979 Mouse
- SwissProt: P15261 Mouse
- Unigene: 549 Mouse
Images
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Standard curve
Mouse IFNGR1 ELISA kit (ab267580) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.