Mouse H60a ELISA Kit (ab267582)
Key features and details
- Sensitivity: 82 pg/ml
- Range: 0.082 ng/ml - 20 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse H60a ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
82 pg/ml -
Range
0.082 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 142.6 132% - 147% Plasma 108 89% - 143% Cell culture media 103 96% - 121% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse H60a ELISA Kit is designed for the quantitative determination of H60a in cell culture supernatants, plasma and serum samples.
Δ Note: Mouse H60a concentration is low in normal serum/plasma, and may not be detectable in this assay.
This assay employs an antibody specific for mouse H60a coated on a 96-well plate. Standards and samples are pipetted into the wells and H60a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse H60a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of H60a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BAFF R, BTC, C5a, CCL6, CD48, CD6, CD80, Chemerin, Clusterin, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, IL-33, IL-7 R alpha, Kremen-1, Limitin, Lipocalin-2, LOX-1, Lungkine, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40 Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Mouse H60a coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Mouse H60a Detection Antibody 2 vials Mouse H60a Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
H60 (histocompatibility 60), together with retinoic acid early inducible (Rae-1) are murine ligands for the lectin-like receptor NKG2D. They are distant relatives of major histocompatibility complex class I molecules. Ectopic expression confers target susceptibility to NK cell attack. -
Cellular localization
Type I membrane protein -
Alternative names
- histocompatibility 60
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Database links
- Entrez Gene: 15101 Mouse
- SwissProt: O77968 Mouse
- SwissProt: Q3TDZ7 Mouse
- Unigene: 2828369 Mouse
Images
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Standard curve
Mouse H60a ELISA kit (ab267582) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.