Mouse GITRL ELISA Kit (ab267573)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse GITRL ELISA Kit
See all TNFSF18/GITRL kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
4 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99 91% - 107% Plasma 98 91% - 103% Cell culture media 105 97% - 113% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse GITRL ELISA Kit is designed for the quantitative determination of GITRL in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for mouse GITRL coated on a 96-well plate. Standards and samples are pipetted into the wells and GITRL present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse GITRL antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GITRL bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30L, CD30, CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Mouse GITRL coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Mouse GITRL Detection Antibody 2 vials Mouse GITRL Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF18/AITR/GITR. Important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. -
Tissue specificity
Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Activation inducible TNF related ligand
- Activation-inducible TNF-related ligand
- AITR ligand
see all -
Database links
- Entrez Gene: 240873 Mouse
- SwissProt: Q7TS55 Mouse
- Unigene: 276823 Mouse
Images
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Example standard curve
These standard curves are for demonstration only. A standard curve must be run with each assay.