Mouse GDH Antibody Pair - BSA and Azide free (ab256677)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse GDH Antibody Pair - BSA and Azide free
See all H6PD/GDH kits -
Assay type
ELISA set -
Range
0.313 ng/ml - 30 ng/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse GDH. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab246532), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse GDH Capture Antibody (unconjugated) 1 x 100µg Mouse GDH Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates. -
Tissue specificity
Present in most tissues examined, strongest in liver. -
Involvement in disease
Defects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS). -
Sequence similarities
In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family.
In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily. -
Cellular localization
Endoplasmic reticulum lumen. Microsomes, endoplasmic reticulum lumen. - Information by UniProt
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Alternative names
- 6 phosphogluconolactonase
- 6-phosphogluconolactonase
- 6PGL
see all -
Database links
- Entrez Gene: 100198 Mouse
- Entrez Gene: 66171 Mouse
- SwissProt: Q8CFX1 Mouse
- SwissProt: Q9CQ60 Mouse
- Unigene: 22183 Mouse
- Unigene: 282284 Mouse
- Unigene: 402679 Mouse
Images
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To learn more about the advantages of recombinant antibodies see here.