Mouse Endostatin ELISA Kit (ab263894)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.33 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Endostatin ELISA Kit
See all Endostatin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 5% Inter-assay Sample n Mean SD CV% Serum 3 4.9% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.33 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 93% - 115% Serum 96 94% - 100% Hep Plasma 90 86% - 96% EDTA Plasma 99 94% - 102% Cit plasma 103 101% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse Endostatin ELISA Kit (ab263894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Endostatin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Endostatin with 2.33 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Endostatin is a cleaved product of the C-terminal domain of collagen XVIII, an endogenous angiogenesis inhibitor. Endostatin localized to wall of vasculature in the elastic fiber and basement membrane where it inhibits endothelial cell migration, in vivo and in vitro. Endostatin induces endothelial cell apoptosis and inhibits tumor growth and impairs blood vessel maturation in wound healing. It is implicated in endothelial cell adhesion and cytoskeletal organization. Mouse endostatin shares a 70, 67, 67% sequence homology with human, rat, and cow, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Endostatin Capture Antibody 1 x 600µl 10X Mouse Endostatin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse Endostatin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. -
Alternative names
- Alpha 1 collagen type 18 (XVIII)(COL18A1)
- Alpha 1 type XVIII collagen
- Antiangiogenic agent
see all -
Database links
- SwissProt: P39061 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Endostatin standard curve in Sample Diluent NS.The Endostatin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Endostatin in mouse serum and plasma samples.The concentrations of Endostatin were measured in duplicates, interpolated from the Endostatin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (EDTA) 1:200 and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endostatin concentration was determined to be 97.87 ng/mL in serum, 72.00 ng/mL in plasma (citrate), 85.87 ng/mL in plasma (EDTA), and 101.37 ng/mL in plasma (heparin).
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Interpolated concentrations of native Endostatin in mouse cell and tissue culture supernatant samples.The concentrations of Endostatin were measured in duplicates, interpolated from the Endostatin standard curves and corrected for sample dilution. Undiluted samples are as follows: heart supernatant 1:8 and kidney supernatant 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Endostatin concentration was determined to be 7.60 ng/mL in heart supernatant and 67.42 ng/mL in kidney supernatant.
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Sandwich ELISA - Mouse Endostatin ELISA Kit (ab263894)To learn more about the advantages of recombinant antibodies see here.
