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Mouse E-Selectin ELISA Kit (CD62E) (ab201279)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8.55 pg/ml
  • Range: 23.4 pg/ml - 1500 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse E-Selectin ELISA Kit (CD62E)
    See all CD62E kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 2.57%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.13%

  • Sample type

    Cell culture supernatant, Serum, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    8.55 pg/ml

  • Range

    23.4 pg/ml - 1500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 85 84% - 89%
    Cell culture media 102 97% - 106%
    Hep Plasma 92 84% - 103%
    EDTA Plasma 94 90% - 100%
    Cit plasma 93 85% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Cow, Pig

  • Product overview

    Mouse E-Selectin ELISA Kit (CD62E) (ab201279) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of E-Selectin (CD62E) protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse E-Selectin (CD62E) with 8.55 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    E-Selectin is a cell-surface glycoprotein that plays a role in immune-adhesion. It is expressed by cytokine-stimulated endothelial cells, and mediates the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. E-selectin is also thought to have a role in capillary morphogenesis. Its structural features include the presence of lectin- and EGF-like domains followed by short consensus repeat domains that contain 6 conserved cysteine residues.

  • Platform

    Microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse E-Selectin ELISA Kit (CD62E) (ab201279)
    Other - Mouse E-Selectin ELISA Kit (CD62E) (ab201279)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of E-Selectin standard curve.
    Example of E-Selectin standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of mouse serum and plasma samples within the working range of the assay.
    Titration of mouse serum and plasma samples within the working range of the assay.

    The 1X dilution for serum was 4%. The 1X dilution for EDTA plasma was 0.5%, and the 1X dilution for citrate and heparin plasma was 2%. Interpolated data values (mean +/- SD, n = 2) are graphed.

  • Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (ab201279)
    Sandwich ELISA - Mouse E-Selectin ELISA Kit (CD62E) (ab201279)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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