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Neuroscience Neurology process Neurogenesis

Mouse DLL1 Matched Antibody Pair Kit (ab215414)

Mouse DLL1 Matched Antibody Pair Kit (ab215414)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Mouse
  • Range: 31.2 pg/ml - 2000 pg/ml

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Overview

  • Product name

    Mouse DLL1 Matched Antibody Pair Kit
    See all DLL1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    10.6 pg/ml
  • Range

    31.2 pg/ml - 2000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse DLL1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse DLL1.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208342. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: IA, ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse DLL1 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse DLL1 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse DLL1 Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Developmental Biology
    • Embryogenesis
    • Somatogenesis
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular
  • Function

    Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • Tissue specificity

    Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • Sequence similarities

    Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • Post-translational
    modifications

    Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession O00548 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Delta
    • Delta (Drosophila) like 1
    • Delta like 1
    • Delta like 1 (Drosophila)
    • Delta like 1 protein
    • Delta like protein 1
    • Delta like protein 1 precursor
    • Delta, Drosophila, homolog of 1
    • Delta-like protein 1
    • Delta1
    • DL1
    • DLL 1
    • DLL1
    • DLL1_HUMAN
    • Drosophila Delta homolog 1
    • H-Delta-1
    • OTTHUMP00000017690
    see all
  • Database links

    • Entrez Gene: 13388 Mouse
    • SwissProt: Q61483 Mouse
    • Unigene: 4875 Mouse

    Images

    • Mouse DLL1 standard curve.
      Mouse DLL1 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Mouse DLL1 Matched Antibody Pair Kit (ab215414)
      Sandwich ELISA - Mouse DLL1 Matched Antibody Pair Kit (ab215414)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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