Mouse CYR61 ELISA Kit (ab253223)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.1 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CYR61 ELISA Kit
See all CYR61/CCN1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3% Inter-assay Sample n Mean SD CV% Serum 5 3.1% -
Sample type
Cell culture supernatant, Serum, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.1 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 89% - 94% Serum 112 108% - 115% Cell culture media 88 83% - 95% EDTA Plasma 127 125% - 133% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CYR61 ELISA Kit (ab253223) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CYR61 protein in cell culture media, cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CYR61 with 10.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CYR61 (Cysteine-rich Angiogenic Inducer 61), also known as CCN Family Member 1 (CCN1) or Insulin-Like Growth Factor-Binding Protein 10 (IGFBP10) is a secreted protein that promotes cell proliferation, chemotaxis, angiogenesis, and cell adhesion. CYR61 exhibits both tumorigenic and tumor suppressor properties, depending in part on the localization.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CYR61 Capture Antibody 1 x 600µl 10X Mouse CYR61 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Mouse CYR61 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. -
Sequence similarities
Belongs to the CCN family.
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 3CH61
- CCN 1
- CCN family member 1
see all -
Database links
- Entrez Gene: 16007 Mouse
- SwissProt: P18406 Mouse
- Unigene: 1231 Mouse
Images
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Other - Mouse CYR61 ELISA Kit (ab253223)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CYR61 standard curve in Sample Diluent 50BP.The CYR61 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CYR61 standard curve in Sample Diluent NS.The CYR61 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CYR61 in mouse serum, plasma (EDTA), and cell culture supernatant samples.The concentrations of CYR61 were measured in duplicates, interpolated from the CYR61 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 100%, and NIH/3T3 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CYR61 concentration was determined to be 209.0 pg/mL in neat serum, 119.6 pg/mL in neat plasma (EDTA), and 137.3 pg/mL in neat NIH/3T3 cell culture supernatant.
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Sandwich ELISA - Mouse CYR61 ELISA Kit (ab253223)To learn more about the advantages of recombinant antibodies see here.
