Mouse Chromogranin A ELISA Kit (ab264631)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 93 pg/ml
- Range: 132.81 pg/ml - 8500 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Chromogranin A ELISA Kit
See all Chromogranin A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.1% Inter-assay Sample n Mean SD CV% Serum 3 2.8% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
93 pg/ml -
Range
132.81 pg/ml - 8500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 95% - 113% Serum 100 93% - 107% Cell culture media 85 74% - 100% Hep Plasma 116 110% - 123% EDTA Plasma 102 94% - 111% Cit plasma 101 96% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Chromogranin A ELISA Kit (ab264631) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Chromogranin A protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Chromogranin A with 93 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Chromogranin A (CHGA) a secreted acidic protein and a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. It is processed to number of non-overlapping peptides including Beta-Granin, Pancreastatin, Catestatin and various forms of Serpins. Chromogranin A is a useful serological and immunohistological marker for the presence of neuroendocrine tumors.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Chromogranin A Capture Antibody 1 x 600µl 10X Mouse Chromogranin A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse Chromogranin A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Post-translational
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Alternative names
- beta Granin
- betagranin (N-terminal fragment of chromogranin A)
- catestatin
see all -
Database links
- Entrez Gene: 12652 Mouse
- SwissProt: P26339 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Chromogranin A standard curve in Sample Diluent NS.The Chromogranin A standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Chromogranin A in mouse serum, plasma and cell culture supernatant samples.The concentrations of Chromogranin A were measured in duplicates, interpolated from the Chromogranin A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2%, plasma (citrate) 2%, plasma (EDTA) 1.5%, plasma (heparin) 2% and pancreas tissue culture supernatant 12%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Chromogranin A concentration was determined to be 367 ng/mL in neat serum, 256 ng/mL in neat plasma (citrate), 443 ng/mL in neat plasma (EDTA), 203 ng/mL in neat plasma (heparin) and 53 ng/mL in neat pancreas tissue culture supernatant.
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Sandwich ELISA - Mouse Chromogranin A ELISA Kit (ab264631)To learn more about the advantages of recombinant antibodies see here.
