Mouse CD30 ELISA Kit (ab113341)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 12.29 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CD30 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
12.29 pg/ml - 3000 pg/mlRecovery
76 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 77.29 69% - 87% Serum 75.99 68% - 84% Plasma 74.88 67% - 83% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: MouseProduct overview
Abcam’s CD30 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CD30 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CD30 coated on a 96- well plate. Standards and samples are pipetted into the wells and CD30 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse CD30 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD30 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CD30 (lyophilized) 2 vials CD30 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CD30 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Database links
- SwissProt: Q60846 Mouse
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse CD30 (lyophilized) 2 vials CD30 Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse CD30 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Database links
- SwissProt: Q60846 Mouse
Images
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Representative Standard Curve
Representative Standard Curve using ab113341
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Representative Standard CurveRepresentative Standard Curve using ab113341