Mouse c-Kit ELISA Kit (CD117) (ab203361)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 30 pg/ml
- Range: 0.23 ng/ml - 15 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse c-Kit ELISA Kit (CD117)
See all c-Kit kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 2.6% Inter-assay Sample n Mean SD CV% Overall 3 5.4% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
30 pg/ml -
Range
0.23 ng/ml - 15 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 92% - 94% Serum 118 115% - 120% Hep Plasma 109 104% - 114% EDTA Plasma 105 93% - 144% Cit plasma 110 103% - 119% Mouse brain extract 104 103% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Sheep, Goat, Cow, Pig -
Product overview
Mouse c-Kit ELISA Kit (CD117) (ab203361) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of c-Kit (CD117) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse c-Kit (CD117) with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
c-Kit (CD117) is a member of the type III subfamily of receptor tyrosine kinases and is the cellular receptor for stem cell factor. Kit is essential for cell survival and proliferation, stem cell maintenance, mast cell development, melanogenesis, cellular migration, hematopoiesis, and gametogenesis. Mutations in human c-Kit are associated with gastrointestional stromal tumors, piebaldism, acute myelogenous leukemia, and mast cell disease.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse c-Kit Capture Antibody 1 x 600µl 10X Mouse c-Kit Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse C-Kit Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. -
Cellular localization
Cell Membrane; single-pass type I membrane protein -
Alternative names
- CD117
- Mast/stem cell growth factor receptor precursor
- PBT
see all -
Database links
- Entrez Gene: 16590 Mouse
- SwissProt: Q7TS86 Mouse
- SwissProt: Q6QJB8 Mouse
Images
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Other - Mouse c-Kit ELISA Kit (CD117) (ab203361)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of c-KIT standard curve prepared in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of c-KIT standard curve prepared in 1X Cell Extraction buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of c-Kit in mouse plasma (EDTA, heparin and citrate).The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of c-Kit in mouse brain and lung extract.The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Interpolated values of c-Kit are plotted based on an extract load of 200 µg/mL.
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Sandwich ELISA - Mouse c-Kit ELISA Kit (CD117) (ab203361)To learn more about the advantages of recombinant antibodies see here.
