Mouse BCA1 Matched Antibody Pair Kit (ab218172)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 31.25 pg/ml - 2000 pg/ml
Overview
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Product name
Mouse BCA1 Matched Antibody Pair Kit
See all BCA1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
5.6 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse BCA1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse BCA1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab212167. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse BCA1 Capture Antibody 2 x 50µg 1 x 50µg Mouse BCA1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse BCA1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. -
Tissue specificity
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
see all -
Database links
- Entrez Gene: 55985 Mouse
- SwissProt: O55038 Mouse
- Unigene: 10116 Mouse
Images
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Mouse BCA1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse BCA1 Matched Antibody Pair Kit (ab218172)To learn more about the advantages of recombinant antibodies see here.
