Antibodies, Proteins, Kits and Reagents for Life Science

Mouse BCA1 Matched Antibody Pair Kit (ab218172)

Key features and details

Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
For economical ELISA and ELISA-based assay development
Reacts with: Mouse
Range: 31.25 pg/ml - 2000 pg/ml

Product name

Mouse BCA1 Matched Antibody Pair Kit
See all BCA1 kits
Detection method

Colorimetric
Assay type

ELISA set
Sensitivity

5.6 pg/ml
Range

31.25 pg/ml - 2000 pg/ml
Species reactivity

Reacts with: Mouse
Product overview

Mouse BCA1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse BCA1.

Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.

Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).

For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab212167. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
Tested applications

Suitable for: ELISAmore details
Platform

Reagents

Storage instructions

Store at -20°C. Please refer to protocols.

Components	10 x 96 tests	5 x 96 tests
Mouse BCA1 Capture Antibody	2 x 50µg	1 x 50µg
Mouse BCA1 Detector Antibody	2 x 12.5µg	1 x 12.5µg
Mouse BCA1 Lyophilized Protein	2 vials	1 vial

Research areas
Function

Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
Tissue specificity

Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
Sequence similarities

Belongs to the intercrine alpha (chemokine CxC) family.
Cellular localization

Secreted.
Target information above from: UniProt accession O43927 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
- B cell-attracting chemokine 1
- B lymphocyte chemoattractant
- B-cell chemoattractant
- B-cell-attracting chemokine 1
- B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
- BCA-1
- BLC
- BLR1L
- C-X-C motif chemokine 13
- Chemokine (C-X-C motif) ligand 13
- Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
- Chemokine, CXC motif, ligand 13
- CXC chemokine BLC
- CXCL13
- CXL13_HUMAN
- SCYB13
- Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
- Small inducible cytokine B13
- Small inducible cytokine subfamily B, member 13
- Small-inducible cytokine B13
see all
Database links
- Entrez Gene: 55985 Mouse
- SwissProt: O55038 Mouse
- Unigene: 10116 Mouse

Mouse BCA1 standard curve.

Standard calibration curve. Background subtracted values are graphed.
Sandwich ELISA - Mouse BCA1 Matched Antibody Pair Kit (ab218172)

To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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