Mouse AGT ELISA Kit (ab245718)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 49.9 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse AGT ELISA Kit
See all Angiotensinogen kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 3% Inter-assay Sample n Mean SD CV% serum 3 1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
49.9 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 103 99% - 105% Serum 112 107% - 115% Hep Plasma 105 92% - 112% EDTA Plasma 111 105% - 115% Cit plasma 112 110% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse AGT ELISA Kit (ab245718) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AGT protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse AGT with 49.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse AGT Capture Antibody 1 x 600µl 10X Mouse AGT Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BR 1 x 6ml Mouse AGT Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-1 (angiotensin 1-10). Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2 (angiotensin 1-8). Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3 (angiotensin 2-8), angiotensin-4 (angiotensin 3-8). Angiotensin 1-7 is cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2.
Angiotensin-2 acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone.
Angiotensin-3 stimulates aldosterone release.
Angiotensin 1-7 is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects (By similarity). Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Genetic variations in AGT are a cause of susceptibility to essential hypertension (EHT) [MIM:145500]. Essential hypertension is a condition in which blood pressure is consistently higher than normal with no identifiable cause.
Defects in AGT are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsBeta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aangiotensinogen (serpin peptidase inhibitor clade A member 8)
- AGT
- AI265500
see all -
Database links
- Entrez Gene: 11606 Mouse
- SwissProt: P11859 Mouse
- Unigene: 301626 Mouse
Images
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Other - Mouse AGT ELISA Kit (ab245718)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse AGT standard curve in Sample Diluent NS.The AGT standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native AGT in mouse serum and plasma samples.The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:2,000, plasma (citrate) 1:2,000, plasma (EDTA) 1:2,000, and plasma (heparin) 1:2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AGT concentration was determined to be 13.4 µg/mL in serum, 9.1 µg/mL in plasma (citrate), 8.8 µg/mL in plasma (EDTA), and 12.5 µg/mL in plasma (heparin).
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Interpolated concentrations of native AGT in mouse liver supernatant samples.The concentrations of AGT were measured in duplicates, interpolated from the AGT standard curves and corrected for sample dilution. Undiluted samples are as follows: mouse liver supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AGT concentration was determined to be 70.7 ng/mL in mouse liver supernatant. -
Sandwich ELISA - Mouse AGT ELISA Kit (ab245718)To learn more about the advantages of recombinant antibodies see here.
