Monkey PD-L1 [28-8] ELISA Kit (ab279408)
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Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15.85 pg/ml
- Range: 32.81 pg/ml - 2100 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey PD-L1 [28-8] ELISA Kit
See all PD-L1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.2% Inter-assay Sample n Mean SD CV% Serum 3 9.1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15.85 pg/ml -
Range
32.81 pg/ml - 2100 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 111 104% - 115% Serum 103 88% - 116% Hep Plasma 89 84% - 94% EDTA Plasma 109 97% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Monkey -
Product overview
Monkey PD-L1 [28-8] ELISA kit (ab279408) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Monkey PD-L1 [28-8] protein in monkey serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey PD-L1 [28-8] with 15.85 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
PD-L1 (also known as CD274 or B7-H1) is a membrane bound glycoprotein involved in regulation of the immune system. PD-L1 is expressed on a variety of inflammatory-activated cells as well as some carcinomas and in melanoma. PD-L1 binds to PD-1 and CD80, where it can suppress T cell activation and proliferation as well as induce apoptosis. Levels of PD-L1 are increased in the plasma of cancer patients as well as in cerebrospinal fluid of gliomas. PD-L1 can bind PD-1 in order to regulate T cell apoptosis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Monkey PD-L1 [28-8] ELISA Kit Capture Antibody 1 x 600µl 10X Monkey PD-L1 [28-8] ELISA Kit Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Monkey PD-L1 [28-8] ELISA Kit Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
Tissue specificity
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane and Endomembrane system. - Information by UniProt
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Alternative names
- B7 H
- B7 H1
- B7 homolog 1
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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The PD-L1 standard curve was prepared as described in Section 10.The PD-L1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PD-L1 in cynomolgus serum and stimulated cynomolgus PBMC cell culture supernatant samples.PBMC cell culture supernatant samples were stimulated with 50 ng/ml PMA and 1 µg/ml ionomycin for 24 hours. The concentrations of PD-L1 were measured in duplicates, interpolated from the PD-L1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cynomolgus serum 50% and stimulated cynomolgus PBMC cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 2,281.25 pg/ml in cynomolgus serum and 2,546.31 pg/ml in stimulated cynomolgus PBMC cell culture supernatant.
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Interpolated concentrations of native PD-L1 in rhesus plasma (heparin), rhesus plasma (EDTA), and stimulated rhesus PBMC cell culture supernatant samples.PBMC cell culture supernatant samples were stimulated with 50 ng/ml PMA and 1 µg/ml ionomycin for 24 hours. The concentrations of PD-L1 were measured in duplicates, interpolated from the PD-L1 standard curves and corrected for sample dilution. Undiluted samples are as follows: rhesus plasma (heparin) 50%, rhesus plasma (EDTA) 25%, and stimulated rhesus PBMC cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 860.85 pg/ml in rhesus plasma (heparin), 3,398.46 pg/ml in rhesus plasma (EDTA), and 4,486.45 pg/ml in stimulated rhesus PBMC cell culture supernatant.
