Monkey GM-CSF ELISA Kit (ab252361)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.45 pg/ml
- Range: 15.63 pg/ml - 1000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey GM-CSF ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 2.3% Inter-assay Sample n Mean SD CV% Supernatant 3 3.3% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.45 pg/ml -
Range
15.63 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95 93% - 97% Cell culture media 96 95% - 98% Hep Plasma 96 95% - 98% EDTA Plasma 94 93% - 97% Cit plasma 93 91% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Monkey
Does not react with: Cow -
Product overview
Monkey GM-CSF ELISA Kit (ab252361) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GM-CSF protein in cell culture media, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey GM-CSF with 8.45 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Monkey GM-CSF Capture Antibody 1 x 600µl 10X Monkey GM-CSF Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Monkey GM-CSF Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells. -
Involvement in disease
Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4) [MIM:300770]. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- CD_antigen=CD116
- CD116
- CD116 antigen
see all
Images
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Other - Monkey GM-CSF ELISA Kit (ab252361)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of monkey GM-CSF standard curve in Sample Diluent NS.The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike GM-CSF in Rhesus macaque serum, plasma and cell culture media samples. -
Interpolated concentrations of native GM-CSF in Rhesus macaque PBMC (PMA-stimulated) cell culture supernatant.The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted sample is as follows: PBMC (PMA-stimulated) cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 3726.5 pg/mL in PBMC (PMA-stimulated) cell culture supernatant. GM-CSF was undetectable in unstimulated PBMC cell culture supernatant.
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Sandwich ELISA - Monkey GM-CSF ELISA Kit (ab252361)To learn more about the advantages of recombinant antibodies see here.
