Monkey Galectin-3 ELISA Kit (LGALS3) (ab269556)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 115 pg/ml
- Range: 208.9 pg/ml - 6000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey Galectin-3 ELISA Kit (LGALS3)
See all Galectin 3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.2% Inter-assay Sample n Mean SD CV% Serum 3 2.6% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
115 pg/ml -
Range
208.9 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 87 83% - 90% Serum 105 100% - 110% Cell culture media 83 81% - 85% Hep Plasma 109 108% - 110% EDTA Plasma 106 102% - 110% Cit plasma 117 116% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Monkey -
Product overview
Monkey Galectin-3 ELISA Kit (LGALS3) (ab269556) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Galectin-3 (LGALS3) protein in edta plasma, hep plasma, serum, cell culture media, cit plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey Galectin-3 (LGALS3) with 115 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Galectin-3 (Gal-3, Galactoside-binding protein, Mac-2 antigen) is a member of the galectin family of carbohydrate-binding proteins. Galectin-3 is active intracellularly and extracellularly. It is widely expressed, including by fibroblasts and macrophages. Intracellularly, Galectin-3 can function in the nucleus as a pre-mRNA splicing factor and in the cytoplasm it has roles in endocytosis, exocytosis and apoptosis. Extracellularly, Galectin-3 is often pro-inflammatory and is involved in innate immunity. Galectin-3 can be monomeric or form higher order homo and hetero oligomers.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Monkey Galectin-3 (LGALS3) Capture Antibody 1 x 600µl 10X Monkey Galectin-3 (LGALS3) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Monkey Galectin-3 (LGALS3) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. -
Tissue specificity
A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Nucleus. Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface. - Information by UniProt
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Alternative names
- 35 kDa lectin
- Carbohydrate binding protein 35
- Carbohydrate-binding protein 35
see all
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of monkey Galectin-3 standard curve in Sample Diluent NS.The Galectin-3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Galectin-3 in Rhesus Macaque monkey serum and plasma samples.The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 4%, plasma (citrate) 4%, plasma (EDTA) 4% and plasma (heparin) 4%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 93 ng/mL in neat serum, 111 ng/mL in neat plasma (citrate), 93 ng/mL in neat plasma (EDTA) and 88 ng/mL in neat plasma (heparin).
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Interpolated concentrations of native Galectin-3 in Rhesus Macaque monkey PBMC cell culture supernatant cultured for 24 hours.The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 57%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 7.5 ng/mL in PBMC supernatant.
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Sandwich ELISA - Monkey Galectin-3 ELISA Kit (LGALS3) (ab269556)To learn more about the advantages of recombinant antibodies see here.
