Methylene Blue Stain Solution ab246830 has been optimized to give outstanding results when used in various procedures such as the Ziehl-Neelsen, Fite's, and Gram stain. The stain is also suggested for use in staining a variety of bacteria (Corynebacterium diphtheria, Haemophilus influenzae, Neisseria, etc.) and leukocytes. This reagent produces dark blue nuclei with very light blue cytoplasm.

Nuclei: Dark Blue
Cytoplasm: Light Blue
Corynebacterium Diphtheria: Blue Granules
Escherichia Coli: Blue Rods
Streptococcus Pyogenes: Blue Cocci

Control: Cytology Smear. Mucus Smear. Fecal Smear. 

Procedure:

1. Deparaffinize sections if necessary and hydrate to distilled water.

2. Apply adequate Methylene Blue Solution to completely cover tissue section and incubate for 1-3 minutes.

3. Rinse slide using absolute alcohol.

4. Dehydrate in 2 changes of absolute alcohol.

5. Clear, and mount in synthetic resin.

Alternative: After staining, rinse briefly in distilled water (2-3 dips), allow slide to air dry, clear in xylene or xylene replacement, and mount in synthetic resin.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.