Membrane Integrity WB Antibody Cocktail (ab110414)
Key features and details
- Assay type: Quantitative
Overview
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Product name
Membrane Integrity WB Antibody Cocktail -
Assay type
Quantitative -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Product overview
ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:
Outer Membrane - Porin ab14734 (MSA03)
Intermembrane Space - Cytochrome c ab110325 (MSA06)
Inner Membrane - Complex Va ab110273 (MS502) and Complex III Core 1 ab110252 (MS303)
Matrix Space - Cyclophilin D ab110324 (MSA04)This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D.
Cocktail Antibodies:
- Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
Amount: 50µg
Working concentration: 2µg/ml - Mouse monoclonal [E11AE12BD4] to Cyclophilin 40 (ab110324):
Amount: 50µg
Working concentration: 2µg/ml - Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I (ab110252):
Amount: 50µg
Working concentration: 2µg/ml - Mouse monoclonal [37BA11] to Cytochrome C (ab110325):
Amount: 5µg
Working concentration: 0.1µg/ml - Mouse monoclonal [7H10BD4F9] to ATP5A (ab110273):
Amount: 100µg
Working concentration: 4µg/ml
- Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
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Tested applications
Suitable for: WBmore details
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 255 µg Cocktail of 5 Antibodies 1 x 255µg Rat liver mitochondria control 1 x 50µg -
Research areas
Images
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Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414)All lanes : Membrane Integrity WB Antibody Cocktail (ab110414)
Lane 1 : Rat liver mitos prep #1
Lane 2 : Rat liver mitos prep #2
Lysates/proteins at 10 µg per lane.
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Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414) This image is courtesy of an anonymous AbreviewSample: Mouse whole cell lysate (3T3 SA cell line)
Loading amount: 20µg
Gel Running conditions: reduced denaturating (4-12% gradient gel)
Blocking step: 30 min in 5% milk at room temperature
Dilution: 1/1000
Incubation time: Overnight at 4°C or 1 hour at room temperature
Secondary antibody: Goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
Detection: ECL