MCM4 overexpression 293T lysate (whole cell) (ab94302)
Overview
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Product name
MCM4 overexpression 293T lysate (whole cell) -
General notes
ab94302 is a 293T cell transfected lysate in which Human MCM4 has been transiently over-expressed using a pCMV-MCM4 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Similarity: Belongs to the MCM family. Contains 1 MCM domain.
Images
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SDS-PAGE - MCM4 overexpression 293T lysate (whole cell) (ab94302)ab94302 at 15μg/lane on an SDS-PAGE gel
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Western blot - MCM4 overexpression 293T lysate (whole cell) (ab94302)All lanes : Anti-MCM4 antibody (ab88546) at 1/500 dilution
Lane 1 :MCM4 overexpression 293T lysate (whole cell) (ab94302)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
