Magnetic Conjugation Kit (ab269890)
Overview
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Product name
Magnetic Conjugation Kit -
Product overview
Magnetic Conjugation Kit (ab269890) allows conjugation of antibodies to specially treated magnetic particles without the need for extensive optimisation of the conjugation reaction.
The 0.5 μm specially treated iron oxide magnetic particles offer you the right balance between high surface area and fast separation. The binding capacity of our magnetic particles is greater than other magnetic particles on the market, and twice that of leading competitors. This allows you to reach the desired sensitivity with fewer particles and therefore minimize the risk of nonspecific binding.
Covalent attachment of antibodies to magnetic particles is ideal for immunoprecipitation experiments and provides a more stable alternative to noncovalent attachment or antibodies using protein A or protein G resin.
Features and Benefits:
Quick and easy to use – save time, no special knowledge required
High binding capacity – achieve high sensitivity with less material
High coupling efficiency – No antibody loss during conjugation
Covalent bond – Highly stable conjugates
No extensive optimisation required – Save precious antibody
Scalable – Easy transfer from R&D to manufacturing
Freeze dried – Long shelf life
Specifications
Core: Iron oxide
Mean diameter: 0.5 µm
Binding capacity: ≥70 µg of Goat anti-rabbit antibody per mg of beads
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Notes
This product is manufactured by Expedeon, an Abcam company. It was previously called Magnetic Conjugation Kit. Expedeon product code 1300-0005 (3 reaction mini kit) is the same as the 3 x 20 µg size of this product, code 1300-0010 (10 reaction mini kit) is the same as the 10 x 20 µg size, and code 1300-0015 (1 reaction midi kit) is the same as the 1 x 200 µg size of this product.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 3 x 20 µg 10 x 20 µg 1 x 200 µg 200 µg ab273924 - Mag Antibody Diluent 1 x 1500µl 1 x 1500µl 1 x 4ml 1 x 1500µl ab273922 - Mag Quencher 1 x 1500µl 1 x 1500µl 1 x 3ml 1 x 1500µl ab273921 - Mag Storage Buffer 1 x 5ml 1 x 5ml 1 x 15ml 1 x 5ml ab273923 - Mag Wash Buffer 1 x 1500µl 1 x 1500µl 1 x 5ml 1 x 1500µl ab273925 - Magnetic particles vial 3 x 20µg 10 x 20µg 1 x 200µg 10 x 20µg
Images
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How The Kit Works
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Rabbit IgG immunoprecipitation and detection by Western blot.25 μg and 5 μg Goat anti-rabbit antibody were added to each of two Mini vials of magnetic particles, and to 25 μL of 1% magnetic beads from a leading competitor, and processed as per the respective protocols. The conjugates were used to immunoprecipitate rabbit IgG from rabbit serum. The eluted samples were run by SDS-PAGE and analysed by Western blot against rabbit IgG. Abcam’s Magnetic Conjugation Kit has a greater binding capacity and coupling efficiency than the competitor products, and allows you to detect the antigen of interest with fewer reagents. Lane 1: Rabbit IgG control; Lanes 2 and 4: Competitor; Lanes 3 and 5: Magnetic Conjugation Kit (ab269890).
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Magnetic Conjugation Kit binding capacity.Goat anti-rabbit antibody was conjugated to 25 μL of 1% magnetic particles from Magnetic Conjugation Kit (ab269890) (1h procedure), and to magnetic particles from a leading competitor (4h procedure) as per the respective protocols. The quantity of IgG added to the magnetic particle Abcam’s Magnetic Conjugation Kit demonstrates a considerably greater binding capacity.
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Rabbit IgG immunoprecipitation.Goat anti-rabbit was labeled with four different batches of magnetic beads. The conjugates were tested in parallel on a Rabbit IgG immunoprecipitation. The bound Rabbit IgG was eluted and quantified by 280nm absorbance.