Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab273332)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Cell Lysate, Other biological fluids, Tissue Lysate
Overview
-
Product name
Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab273332) utilizes LPC-specific enzymes to generate an intermediate that then reacts with a probe, yielding a signal that can be quantified either colorimetrically or fluorometrically, and is proportional to the amount of LPC present in the sample.
When used as described, the assay is capable of detecting as little as 10 pmole of Lysophosphatidylcholine.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2X Lipid Resuspension Buffer 2 x 1ml LPC Assay Buffer 1 x 25ml LPC Developer Lyophilized 1 vial LPC Enzyme Mix Lyophilized 1 vial LPC Probe (in DMSO) 1 x 200µl LPC Standard (0.5 µmol) Lyophilized 1 vial
Images
-
Example data
Example data.
LPC Standard curve, fluorometric.
-
Example dataExample data.
LPC Standard curve, colorimetric.
-
Example dataExample data.
Determination of total LPC concentration in pooled human serum. Sample was analyzed according to the kit protocol. Values were determined with fluorometric measurements and are mean ± standard deviation of at least three independent determinations.
-
Example dataExample data.
Specificity of the assay: PC = phosphatidylcholine, PA = phosphatidic acid, SM = sphingomyelin, LPC = lysophosphatidylcholine. 1 nmole of each was tested in the fluorometric assay.