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LY 294002, PI3-kinase inhibitor (ab120243)

Key features and details

  • PI3-kinase inhibitor
  • CAS Number: 154447-36-6
  • Purity: > 99%
  • Soluble in DMSO to 25 mM and in ethanol to 5 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    LY 294002, PI3-kinase inhibitor

  • Description

    PI3-kinase inhibitor

  • Biological description

    A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).

  • Purity

    > 99%

  • CAS Number

    154447-36-6

  • Chemical structure

    Chemical Structure

Properties

Images

  • Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
    Western blot - LY 294002, PI3-kinase inhibitor (ab120243)
    Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.

    Lane 1: Marker
    Lane 2: A431 lysate starved
    Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
    Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
    Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)

    Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution

    Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.

    Observed bands:
    RSK1p90 (pS380) band size: 83 kDa
    AKT1 (pS473) band size: 56 kDa
    ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
    RPS6 (pS235/236) band size: 31 kDa
    Rab11a band size: 24 kDa
    Unknown band: 130kDa

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