Live and Dead Cell Assay Kit (Calcein AM, 7-AAD) (ab270789) combines Calcein AM with 7-aminoactinomycin D (7-AAD) to allow for easy and simultaneous labeling of live, membrane compromised, and dead cells within a single sample.

The large quantum yield of Calcein dyes enables them to be readily detected within widely used applications such as flow cytometers and fluorescence microscopes. The degree of fluorescence correlates with relative cell viability status. For microscopy usage, Hoechst 33342 is included with the kit to concurrently label nuclei after labeling with Calcein. Because Calcein alone will detect cells that are alive, but some of which could possess compromised cell membrane structure and thus be in the process of dying, it is possible to obtain an overly positive picture of the overall health status of the cell population.

Sample size and Calcein AM staining concentration needed may vary based on the experimental conditions and the instrument used for analysis. Each investigator should adjust the amount of Calcein AM to accommodate the particular cell line and research conditions.

NOTE: Tests per vial range from 25 to 250 depending on application.