Lactulose Assay Kit (Fluorometric) (ab196986)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Milk
Overview
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Product name
Lactulose Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Milk -
Assay type
Quantitative -
Range
0.01 nmol/well - 1 nmol/well -
Product overview
Lactulose Assay Kit (Fluorometric) (ab196986) is a simple, sensitive and convenient assay to quantify lactulose in non-biological samples such as pharmaceuticals, foods, and dairy products. Lactulose present in the sample is hydroyzed by the Lactulose Enzyme Mix to form galactose and fructose. Production of fructose can be measured in a fluorescence plate reader (Ex/Em = 535/587 nm) and the amount of fructose is directly proportional to the amount of lactulose present in the sample. On the other hand, traditional lactulose assays are based on detecting the amount of glucose formed from fructose, leading to incresaed background level in the assay.
The linear range of the assay is between approximately 10 pmol and 1 nmol of lactulose.
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Notes
Lactulose is a synthetic disaccharide composed of galactose and fructose. It is closely related to lactose which is a disaccharide consisting of galactose and glucose. Lactulose is not absorbed by the gut and it is therefore commonly used as a laxative and for hepatic encephalopathy. It can also be found in heated milk since it is easily formed by isomerization of lactose at elevated temperatures or in alkaline solutions.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Enhancement Solution 1 x 1.5ml Hydrolysis Enzyme Mix (lyophilized) Blue 1 vial Lactulose Enzyme Mix 1 vial Lactulose Hydrolysis Buffer 1 x 25ml Lactulose Reaction Buffer 1 x 25ml Lactulose Standard (100 mM) 1 x 80µl PicoProbe Blue 1 x 300µl
Images
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Standard curve of lactulose and results obtained in milk
Left: Standard Curve of Lactulose before and after enhancement (not corrected for background). Right: Results obtained for milk: 100 µL were treated with Carrez Clarification Reagent, then 10 µL were diluted 1:20 with Lactulose Hydrolysis Buffer and analyzed following this protocol.
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Time course standard curve for lactulose detectionTime course of Standard Curve of 0-1 nmol lactulose before enhancement and signal after enhancement.