Ketone Body Assay Kit (ab272541)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Other biological fluids, Plasma, Serum, Urine
Overview
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Product name
Ketone Body Assay Kit -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids -
Assay type
Quantitative -
Range
0.12 mM - 8 mM -
Product overview
Ketone Body Assay Kit (ab272541) is a simple, direct and automation-ready procedure for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH). This ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations.
Sensitive and accurate: Uses 10 μL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay.
Convenient: The procedure involves adding a single working reagent, and reading the optical density at room temperature.
High-throughput: Can be automated as a high-throughput 96-well plate assay for many samples per day.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests AcAc Buffer 1 x 20ml AcAc Reagent 1 vial AcAc Standard 1 x 200µl BOH Buffer 1 x 20ml BOH Reagent 1 x 1ml BOH Standard 1 x 200µl HBDH Enzyme 1 x 120µl
Images
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Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Example of Acetoacetic Acid (AcAc) and 3-Hydroxybutyric Acid (BOH) standard curve.