Hydralazine hydrochloride, DNA methylation inhibitor. (ab120863)
Key features and details
- DNA methylation inhibitor. Antihypertensive agent.
- CAS Number: 304-20-1
- Purity: > 99%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
Hydralazine hydrochloride, DNA methylation inhibitor. -
Description
DNA methylation inhibitor. Antihypertensive agent. -
Biological description
Inhibits DNA methylation by inhibition of MAPK (EC50 = 2.96 μM). Multiple, direct effects on vascular smooth muscle; hyperpolarization, inhibition of IP3-induced release of Ca2+ and formation of NO leading to cGMP-mediated vasodilation. DNMT inhibitor (IC50 = 2 µM).
-
Purity
> 99% -
CAS Number
304-20-1 -
Chemical structure
Properties
-
Chemical name
1-Hydrazinylpthalazine hydrochloride -
Molecular weight
196.64 -
Molecular formula
C8H8N4.HCl -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
Source
Synthetic
-
Research areas
Images
-
Functional Studies - Hydralazine hydrochloride, DNA methylation inhibitor. (ab120863)
Jurkat cells were incubated at 37°C for 5 days with vehicle control (0 µM) and different concentrations of hydralazine hydrochloride (ab120863). Decreased expression of DNMT1 (ab19905) in Jurkat cells correlates with an increase in hydralazine hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab19905 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.