Human Zinc Alpha 2 Glycoprotein ELISA kit (ab277419)
Key features and details
- Sensitivity: 0.04 ng/ml
- Range: 0.041 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Zinc Alpha 2 Glycoprotein ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.04 ng/ml -
Range
0.041 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 78.73 73% - 84% Plasma 79.34 76% - 85% Cell culture media 74.51 70% - 78% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Zinc Alpha 2 Glycoprotein ELISA Kit (ab277419) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Zinc Alpha 2 Glycoprotein in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Zinc Alpha 2 Glycoprotein coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Zinc Alpha 2 Glycoprotein present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Zinc Alpha 2 Glycoprotein antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Zinc Alpha 2 Glycoprotein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 900x HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human Zinc Alpha 2 Glycoprotein Antibody 2 vials Anti-Human Zinc Alpha 2 Glycoprotein coated Microplate 1 unit Human Zinc Alpha 2 Glycoprotein standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids. -
Tissue specificity
Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver. -
Sequence similarities
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Alpha 2 glycoprotein 1 zinc
- Alpha 2 glycoprotein 1 zinc binding
- Alpha 2 glycoprotein zinc
see all -
Database links
- Entrez Gene: 563 Human
- Omim: 194460 Human
- SwissProt: P25311 Human
- Unigene: 546239 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.