Human YAP1 ELISA Kit (ab230942)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 40 pg/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human YAP1 ELISA Kit
See all YAP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HeLa extract 5 4.5% Inter-assay Sample n Mean SD CV% HeLa extracy 3 4.5% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
40 pg/ml -
Range
0.31 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 96.4 90% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human YAP1 ELISA Kit (ab230942) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of YAP1 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human YAP1 with 40 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human YAP1 Capture Antibody 1 x 600µl 10X Human YAP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human YAP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3). -
Tissue specificity
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). -
Sequence similarities
Belongs to the YORKIE family.
Contains 2 WW domains. -
Post-translational
modificationsPhosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. -
Cellular localization
Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. - Information by UniProt
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Alternative names
- 65 kDa Yes associated protein
- 65 kDa Yes-associated protein
- COB1
see all -
Database links
- Entrez Gene: 10413 Human
- Omim: 606608 Human
- SwissProt: P46937 Human
- Unigene: 503692 Human
Images
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Other - Human YAP1 ELISA Kit (ab230942)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human YAP1 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native YAP1 in human heart homogenate tissue extractHEK293T cell extract and HELA cell extract. The concentrations of YAP1 were measured in duplicate and interpolated from the YAP1 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YAP1 concentration was determined to be pg/µg in human heart homogenate tissue extract, 7.5 pg/µg in HEK293T cell extract, and 13.7 pg/µg in Hela cell extract.
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Sandwich ELISA - Human YAP1 ELISA Kit (ab230942)To learn more about the advantages of recombinant antibodies see here.
