Human Visfatin ELISA Kit (ab264623)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 80 pg/ml
- Range: 234.38 pg/ml - 15000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Visfatin ELISA Kit
See all Visfatin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 2.3% Inter-assay Sample n Mean SD CV% Extract 3 7.3% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
80 pg/ml -
Range
234.38 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 93 90% - 96% Cell Lysate 104 97% - 114% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Visfatin ELISA Kit (ab264623) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Visfatin protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Visfatin with 80 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Visfatin, also known as nicotinamide phosphoribosyltransferase, is the key enzyme in the salvage pathway by which the cell converts nicotinamide to nicotinamide mononucleotide. Along with its enzymatic role, Visfatin is thought to play a pivotal role in B-cell maturation and cell signaling. This protein is highly conserved throughout mammals, subsequently, this kit cross-reacts with both mouse and rat.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Visfatin Capture Antibody 1 x 600µl 10X Human Visfatin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human Visfatin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 1110035O14Rik
- AI314458
- AI480535
see all -
Database links
- Entrez Gene: 10135 Human
- Omim: 608764 Human
- SwissProt: P43490 Human
- Unigene: 489615 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Visfatin standard curve in 1X Cell Extraction Buffer PTR.The Visfatin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Visfatin in human liver tissue extract and K562 cell extract based on a 15 µg/mL and 50 µg/mL extract load.The concentrations of Visfatin were measured in duplicate and interpolated from the Visfatin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Visfatin concentration was determined to be 7,060.81 pg/mL in liver tissue extract and 10,559.80 pg/mL in K562 cell extract.
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Sandwich ELISA - Human Visfatin ELISA Kit (ab264623)To learn more about the advantages of recombinant antibodies see here.
