Human TRMT2A (HTF9C) knockout HeLa cell line (ab265160)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Cervix -
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human TRMT2A knockout HeLa cell line (ab265160)All lanes : Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRMT2A knockout HeLa cell lysate
Lane 3 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab205616 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab205616 Anti-HTF9C/TRMT2A antibody [EPR16485-54] was shown to specifically react with HTF9C/TRMT2A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265160 (knockout cell lysate ab258253) was used. Wild-type and HTF9C/TRMT2A knockout samples were subjected to SDS-PAGE. ab205616 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human TRMT2A knockout HeLa cell line (ab265160)All lanes : Anti-HTF9C/TRMT2A antibody [1G8] (ab230922) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRMT2A knockout HeLa cell lysate
Lane 3 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab230922 observed at 75 kDa. Red - loading control ab181602 observed at 36 kDa.
ab230922 Anti-HTF9C/TRMT2A antibody [1G8] was shown to specifically react with HTF9C/TRMT2A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265160 (knockout cell lysate ab258253) was used. Wild-type and HTF9C/TRMT2A knockout samples were subjected to SDS-PAGE. ab230922 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human TRMT2A knockout HeLa cell line (ab265160)Homozygous: 1 bp insertion in exon 2.