Human TRAIL ELISA Kit (CD253) (ab46074)
Key features and details
- Sensitivity: 64 pg/ml
- Range: 93.75 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml Biotinylated anti-TRAIL Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Plastic Plate Covers 2 units 4 units Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl TRAIL Microplate (12 x 8 well strips) 1 unit 2 units TRAIL Standard (Lyophilized) Yellow 2 vials 4 vials -
Research areas
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Relevance
TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. -
Cellular localization
Cell Membrane; single-pass type II membrane protein. -
Alternative names
- Apo 2 ligand
- APO2L
- CD253
see all -
Database links
- Entrez Gene: 8743 Human
- SwissProt: P50591 Human
Images
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ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)
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ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)TRAIL measured in undiluted biological fluids (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab46074
