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Human TRAIL ELISA Kit (CD253) (ab46074)

Key features and details

  • Sensitivity: 64 pg/ml
  • Range: 93.75 pg/ml - 3000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml
    200X Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7ml 1 x 13ml
    Biotinylated anti-TRAIL Red 1 x 400µl 2 x 400µl
    Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml
    Control Silver 2 vials 4 vials
    HRP Diluent Red 1 x 12ml 1 x 23ml
    Plastic Plate Covers 2 units 4 units
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
    TRAIL Microplate (12 x 8 well strips) 1 unit 2 units
    TRAIL Standard (Lyophilized) Yellow 2 vials 4 vials

  • Research areas

  • Relevance

    TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of TRAIL may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.

  • Cellular localization

    Cell Membrane; single-pass type II membrane protein.

  • Alternative names

    • Apo 2 ligand
    • APO2L
    • CD253
    • Protein TRAIL
    • TL2
    • TNF related apoptosis inducing ligand
    • Tumor necrosis factor ligand superfamily member 10
    see all

  • Database links

    Images

    • ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)
      ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)

      TRAIL measured in undiluted cell culture supernatants from control (-) or stimulated ((P/I); 48 hours with 50 ng x mL-1 PMA (ab120297) and 1 uM ionomycin (ab120116)) Jurkat cells (duplicates +/- SD).

    • ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)
      ELISA: TRAIL (CD253) Human ELISA Kit (ab46074)

      TRAIL measured in undiluted biological fluids (duplicates +/- SD).

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab46074

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