Human TPM3 (Tropomyosin 3) knockout HEK-293T cell line (ab266422)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human TPM3 (Tropomyosin 3) knockout HEK293T cell line (ab266422)All lanes : Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : TPM3 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab113692 observed at 37 kDa. Red - loading control ab52901 observed at kDa.
ab113692 Anti-Tropomyosin 3 antibody [3D5AH3AB4] was shown to specifically react with Tropomyosin 3 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266422 (knockout cell lysate ab258245) was used. Wild-type and Tropomyosin 3 knockout samples were subjected to SDS-PAGE. ab113692 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (ab52901) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human TPM3 knockout HEK293T cell line (ab266422)Homozygous: 1 bp insertion in exon 2
