Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human TNFSF12 Antibody Pair - BSA and Azide free (ab253680)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human TNFSF12 Antibody Pair - BSA and Azide free
    See all TWEAK kits

  • Assay type

    ELISA set

  • Range

    47 pg/ml - 3000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview


    • Human TNFSF12 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human TNFSF12 in sandwich ELISAs and many other pair-based applications.

    • The pair can be used in variety of assays and platforms including but not limited to:

    •      - Sandwich ELISA

    •      - FRET/TR-FRET/HTR

    •      - Meso Scale Discovery® (MSD®)

    •      - Luminex® and bead-based assays

    •      - AlphaLISA®/AlphaScreen®

    •      - DELFIA® immunoassays

    •      - Simoa® and Single Molecule Counting (SMC™) immunoassays

    •      - Multiplex

    • Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

    •      - Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.

    •      - Concentration of ~1 mg/ml as measured by the protein A280 method.

    • Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
    • We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

    • Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

    • Please note:

    • The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

    • The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

    • We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

    •  Antibody properties:

    •  Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

    •  Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

    •  Concentration: ~1 mg/ml

    •  Storage buffer: 100% PBS

    •  Form: Liquid

    •  Isotype: IgG

    •  Recombinant monoclonal antibodies offer several advantages including:

    •      - High batch-to-batch consistency and reproducibility

    •      - Improved sensitivity and specificity

    •      - Long-term security of supply

    •      - Animal-free production

    • For more information see here.

    • Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    • Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

    • Luminex is a trademark of Luminex Corporation, registered in the US and other countries.

    • AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

    • Simoa is a registered trademark of Quanterix, Inc.

    • SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components Identifier 10 x 96 tests
    Human TNFSF12 Capture Antibody (unconjugated) —
    Human TNFSF12 Detector Antibody (unconjugated) —

  • Research areas

  • Function

    Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.

  • Tissue specificity

    Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Alternative names

    • APO 3 ligand
    • APO 3L
    • APO3 ligand
    • APO3/DR3 ligand
    • APO3L
    • DR3LG
    • MGC129581
    • MGC20669
    • secreted form
    • TNF-related weak inducer of apoptosis
    • TNF12_HUMAN
    • TNFSF 12
    • Tnfsf12
    • TNFSF12 protein
    • Tumor necrosis factor (ligand) superfamily member 12
    • Tumor necrosis factor ligand superfamily member 12
    • Tumor necrosis factor superfamily member 12
    • TWEAK
    • UNQ181/PRO207
    see all

  • Database links

    Images

    • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253680)
      Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253680)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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