Human TNFAIP3 knockout A549 cell lysate (ab257114)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263513 - Human TNFAIP3 knockout A549 cell lysate 1 x 100µg ab255554 - Human wild-type A549 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human TNFAIP3 knockout A549 cell lysate (ab257114)Lane 1: Wild-type A549 cell lysate (20µg)
Lane 2: TNFAIP3 knockout A549 cell lysate (20µg)
Lane 3: Wild-type HeLa cell lysate (20µg)
Lane 4: TNFAIP3 knockout HeLa cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - ab92324 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.
ab92324 Anti-TNFAIP3 antibody [EPR2663] was shown to specifically react with TNFAIP3 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line ab266946 (knockout cell lysate ab257114) was used. Wild-type and TNFAIP3 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab92324 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human TNFAIP3 knockout A549 cell lysate (ab257114)Allele-1: 1 bp deletion in exon7
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Sanger Sequencing - Human TNFAIP3 knockout A549 cell lysate (ab257114)Allele-2: 1 bp insertion in exon7
