Human TIMP1 ELISA Kit (ab187394)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TIMP1 ELISA Kit
See all TIMP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.4% Inter-assay Sample n Mean SD CV% Serum 3 0.6% -
Sample type
Cell culture supernatant, Saliva, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
17 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 89 86% - 92% Saliva 84 82% - 87% Serum 89 89% - 90% Hep Plasma 95 94% - 98% EDTA Plasma 89 86% - 91% Cit plasma 97 95% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TIMP1 ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency.
Human TIMP1 ELISA Kit (ab187394) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TIMP1 protein in cit plasma, edta plasma, hep plasma, serum, saliva, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TIMP1 with 17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Tissue inhibitor of metalloproteinases 1 (TIMP1 or Metalloproteinase inhibitor 1) is a widely expressed inhibitor of matrix metalloproteinases (MMPs). It functions by binding non-covalently to MMPs (in a 1:1 stoichiometry) and blocking access of substrate to the MMP active site. TIMP1 expression is induced by pro-inflammatory cytokines. It is a soluble factor found circulating in serum and plasma. TIMP1 appears also have functions that are independent of MMP inhibition, including promoting erythropoiesis and inhibiting apoptosis in B cells.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TIMP-1 Capture Antibody 1 x 600µl 10X Human TIMP-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human TIMP-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
Sequence similarities
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
Post-translational
modificationsThe activity of TIMP1 is dependent on the presence of disulfide bonds. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
Database links
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human TIMP-1 competitor curve comparisonStandard Curve comparison between Human TIMP-1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human TIMP-1 standard curve comparisonStandard Curve comparison between the original Human TIMP-1 SimpleStep ELISA kit and current Human TIMP-1 SimpleStep ELISA kit.
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Example of human TIMP-1 standard curve in Sample Diluent NS.The TIMP-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TIMP-1 in human serum, and plasma samples.The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 95.9 ng/mL in serum, 74 ng/mL in plasma (citrate), 68 ng/mL in plasma (EDTA) and 56 ng/mL in plasma (heparin).
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 92.6 ng/mL with a range of 66.5 – 128.7 ng/mL.
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Interpolated concentrations of native TIMP-1 in human cell culture supernatant samples.The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC cell culture supernatant 20% and stimulated PBMC cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 5 ng/mL in unstimulated and 22 ng/mL in stimulated and undetectable in media.
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Interpolated concentrations of native TIMP-1 in human saliva sample.The concentrations of TIMP-1 were measured in duplicates, interpolated from the TIMP-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 2%, The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP-1 concentration was determined to be 179.4 ng/mL in saliva.
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Sandwich ELISA - Human TIMP1 ELISA Kit (ab187394)To learn more about the advantages of recombinant antibodies see here.
