Human Thrombopoietin ELISA Kit (ab219632)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Thrombopoietin ELISA Kit
See all Thrombopoietin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 5.1% Inter-assay Sample n Mean SD CV% Seum 5 5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 83 81% - 84% Cell culture media 89 84% - 84% Hep Plasma 98 97% - 100% EDTA Plasma 101 96% - 109% Cit plasma 84 80% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Thrombopoietin ELISA Kit (ab219632) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Thrombopoietin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Thrombopoietin with 11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Thrombopoietin is a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. Thrombopoietin acts at a late stage of megakaryocyte development.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Thrombopoietin Capture Antibody 1 x 600µl 10X Human Thrombopoietin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Thrombopoietin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
Involvement in disease
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
Sequence similarities
Belongs to the EPO/TPO family. -
Domain
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C mpl ligand
- C-mpl ligand
- Megakaryocyte colony stimulating factor
see all -
Database links
- Entrez Gene: 7066 Human
- Omim: 600044 Human
- SwissProt: P40225 Human
- Unigene: 1166 Human
Images
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Other - Human Thrombopoietin ELISA Kit (ab219632)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Thrombopoietin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Thrombopoietin standard curve in Sample Diluent 10BP.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Thrombopoietin standard curve in Sample Diluent 25BP.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike Thrombopoietin in human plasma samples.The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) neat, plasma (heparin) neat, plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spike Thrombopoietin in human serum and cell culture media (DMEM + 10% FBS) samples.The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, cell culture media neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Thrombopoietin in human serum and plasma samples.The concentrations of Thrombopoietin were measured in duplicates, interpolated from the Thrombopoietin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum neat, plasma (citrate) neat, plasma (heparin) neat, plasma (EDTA) neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 47.1 pg/mL in neat serum, 79.6 pg/mL in neat plasma (citrate), 109 pg/mL in plasma (heparin), and 54.1 pg/mL in plasma (EDTA). Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.
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Serum from ten individual healthy female human donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable Thrombopoietin concentration was determined to be 60.1 pg/mL with a range of 19.6 – 151 pg/mL. Detectable concentrations are defined as concentrations above the minimum detectable dose (MDD) concentration.
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Sandwich ELISA - Human Thrombopoietin ELISA Kit (ab219632)To learn more about the advantages of recombinant antibodies see here.